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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
12/07/2022 |
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Actualizado : |
12/07/2022 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
KALDS, P.; CRISPO, M.; TESSON, L.; ANEGÓN, I.; CHEN KEY, Y.; WANG, X.; MENCHACA, A. |
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Afiliación : |
PETER KALDS, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; MARTINA CRISPO, Unidad de Biotecnología en Animales de Laboratorio (UBAL), Institut Pasteur de Montevideo, Montevideo, Uruguay.; LAURENT TESSON, INSERM, Centre de Recherche en Transplantation et Immunologie, UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; IGNACIO ANEGÓN, INSERM, Centre de Recherche en Transplantation et Immunologie, UMR 1064, Nantes, France.; YULIN CHEN KEY, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; XIAOLONG WANG, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay. |
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Título : |
Generation of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene. |
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Complemento del título : |
Chapter 16. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Methods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16 |
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ISBN : |
Online: 978-1-0716-2301-5 |
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DOI : |
10.1007/978-1-0716-2301-5_16 |
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Idioma : |
Inglés |
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Contenido : |
Abstract:
The myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal muscle mass, and the translational inhibition of this gene has shown increased muscle mass, generating what is known as ?double-muscling phenotype.? Disruption of the MSTN gene expression using the CRISPR/Cas9 genome-editing system has shown improved muscle development and growth rates in livestock species, including sheep and goats. Here, we describe procedures for the generation of MSTN knockout sheep and goats using the microinjection approach of the CRISPR/Cas9 system, including the selection of targeting sgRNAs, the construction of CRISPR/Cas9 targeting vector, the in vitro examination of system efficiency, the in vivo targeting to generate MSTN knockout founders, the genomic and phenotypic characterization of the generated offspring, and the assessment of off-target effects in gene-edited founders through targeted validation of predicted off-target sites, as well as genome-wide off-target analysis by whole-genome sequencing. Editing the MSTN gene using the CRISPR/Cas9 system might be a rapid and promising alternative to promote meat production in livestock. |
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Palabras claves : |
CRISPR/Cas9; Genome editing; Goats; Knockout; Meat production; Microinjection; MSTN; Sheep; Small ruminants. |
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Thesagro : |
CABRAS; OVEJA; PRODUCCION DE CARNE. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02176naa a2200349 a 4500
001 1063414
005 2022-07-12
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1007/978-1-0716-2301-5_16$2DOI
100 1 $aKALDS, P.
245 $aGeneration of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene.$h[electronic resource]
260 $c2022
520 $aAbstract: The myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal muscle mass, and the translational inhibition of this gene has shown increased muscle mass, generating what is known as ?double-muscling phenotype.? Disruption of the MSTN gene expression using the CRISPR/Cas9 genome-editing system has shown improved muscle development and growth rates in livestock species, including sheep and goats. Here, we describe procedures for the generation of MSTN knockout sheep and goats using the microinjection approach of the CRISPR/Cas9 system, including the selection of targeting sgRNAs, the construction of CRISPR/Cas9 targeting vector, the in vitro examination of system efficiency, the in vivo targeting to generate MSTN knockout founders, the genomic and phenotypic characterization of the generated offspring, and the assessment of off-target effects in gene-edited founders through targeted validation of predicted off-target sites, as well as genome-wide off-target analysis by whole-genome sequencing. Editing the MSTN gene using the CRISPR/Cas9 system might be a rapid and promising alternative to promote meat production in livestock.
650 $aCABRAS
650 $aOVEJA
650 $aPRODUCCION DE CARNE
653 $aCRISPR/Cas9
653 $aGenome editing
653 $aGoats
653 $aKnockout
653 $aMeat production
653 $aMicroinjection
653 $aMSTN
653 $aSheep
653 $aSmall ruminants
700 1 $aCRISPO, M.
700 1 $aTESSON, L.
700 1 $aANEGÓN, I.
700 1 $aCHEN KEY, Y.
700 1 $aWANG, X.
700 1 $aMENCHACA, A.
773 $tMethods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16
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Registro original : |
INIA La Estanzuela (LE) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
25/09/2019 |
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Actualizado : |
10/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
B - 1 |
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Autor : |
VALLVERDÚ, C.; VILA, R.; LORENZO, D.; PAZ, D.; DELLACASSA, E.; DAVIES, P.; VILLAMIL, J.; TOMI, F.; CASANOVA, J.; CAÑIGUERAL, S. |
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Afiliación : |
CAROL VALLVERDÚ,, Universitat de Barcelona / Facultat de Farmàcia. Spain; ROSER VILA, Universitat de Barcelona / Facultat de Farmàcia. Spain.; DANIEL LORENZO, Universidad de la República (UdelaR)/ Facultad de Química.; DANIEL PAZ, Universidad de la República (UdelaR)/ Facultad de Química.; EDUARDO DELLACASSA, Universidad de la República (UdelaR)/ Facultad de Química.; PHILIP DAVIES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOSE MILTON VILLAMIL LUCAS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FELIX TOMI, Équipe de Chimie et Biomasse, Université de Corse, France.; JOSEPH CASANOVA, Équipe de Chimie et Biomasse, Université de Corse, France.; SALVADOR CAÑIGUERAL, Universitat de Barcelona / Facultat de Farmàcia. Spain. |
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Título : |
Composition of a new essential oil type of Lippia alba (Mill.) N.E. Brown from Uruguay. |
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Fecha de publicación : |
2001 |
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Fuente / Imprenta : |
Flavour and Fragrance Journal, 2001, Volume 16, Issue 5, Pages 356-359. |
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DOI : |
10.1002/ffj.1450 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 11 July 2000 / Revised 21 March 2001 / Accepted 24 March 2001. |
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Contenido : |
ABSTRACT.
The essential oil of aerial parts of Salvia guaranitica, grown in a small-scale experimental field in Uruguay, was analysed by GC, GC?MS and 13C-NMR; 49 constituents (86.6% of the total sample) were identified. The major components were found to be sesquiterpene hydrocarbons: germacrene D (25.0%), ?-elemene (20.0%), ?-caryophyllene (8.8%) and ?-bourbonene (6.2%), whereas monoterpenes only reached 4% of the sample analysed.
Copyright © 2005 John Wiley & Sons, Ltd. |
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Palabras claves : |
Essential oil; Germacrene D; Lamiaceae; SALVIA GUARANITICA; Sesquiterpene hydrocarbons. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 01442naa a2200313 a 4500
001 1060218
005 2019-10-10
008 2001 bl uuuu u00u1 u #d
024 7 $a10.1002/ffj.1450$2DOI
100 1 $aVALLVERDÚ, C.
245 $aComposition of a new essential oil type of Lippia alba (Mill.) N.E. Brown from Uruguay.$h[electronic resource]
260 $c2001
500 $aArticle history: Received 11 July 2000 / Revised 21 March 2001 / Accepted 24 March 2001.
520 $aABSTRACT. The essential oil of aerial parts of Salvia guaranitica, grown in a small-scale experimental field in Uruguay, was analysed by GC, GC?MS and 13C-NMR; 49 constituents (86.6% of the total sample) were identified. The major components were found to be sesquiterpene hydrocarbons: germacrene D (25.0%), ?-elemene (20.0%), ?-caryophyllene (8.8%) and ?-bourbonene (6.2%), whereas monoterpenes only reached 4% of the sample analysed. Copyright © 2005 John Wiley & Sons, Ltd.
653 $aEssential oil
653 $aGermacrene D
653 $aLamiaceae
653 $aSALVIA GUARANITICA
653 $aSesquiterpene hydrocarbons
700 1 $aVILA, R.
700 1 $aLORENZO, D.
700 1 $aPAZ, D.
700 1 $aDELLACASSA, E.
700 1 $aDAVIES, P.
700 1 $aVILLAMIL, J.
700 1 $aTOMI, F.
700 1 $aCASANOVA, J.
700 1 $aCAÑIGUERAL, S.
773 $tFlavour and Fragrance Journal, 2001, Volume 16, Issue 5, Pages 356-359.
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